EXBIO/抗颗粒酶B FITC/100测试/1F-634-T100
商品编号：
1F-634-T100
品牌：
EXBIO
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
Human NK cell line YT-INDY-derived granzyme B
Other names
GZMB, HLP, CTLA1, SECT
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden.Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry multicolor intracellular staining of human lymphocytes stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD3 negative Granzyme B positive lymphocytes (red-filled) from CD3 negative Granzyme B negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
General references:
Martin P, Pardo J, Schill N, Joeckel L, Berg M, Froelich CJ, Wallich R, Simon MM: Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics. J Biol Chem. 2010 Apr 15. [Epub ahead of print] PubMed
Kummer JA, Kamp AM, Tadema TM, Vos W, Meijer CJ, Hack CE: Localization and identification of granzymes A and B-expressing cells in normal human lymphoid tissue and peripheral blood. Clin Exp Immunol. 1995 Apr;100(1):164-72. PubMed
Kummer JA, Wever PC, Kamp AM, ten Berge IJ, Hack CE, Weening JJ: Expression of granzyme A and B proteins by cytotoxic lymphocytes involved in acute renal allograft rejection. Kidney Int. 1995 Jan;47(1):70-7. PubMed
Product specific references:
Tak PP, Spaeny-Dekking L, Kraan MC, Breedveld FC, Froelich CJ, Hack CE: The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA). Clin Exp Immunol. 1999 May;116(2):366-70.PubMed
Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Péronne C, de Saint Vis B, Brière F, Bates EE: Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002 Nov 1;100(9):3295-303.PubMed
Sun Y, Schmitz JE, Acierno PM, Santra S, Subbramanian RA, Barouch DH, Gorgone DA, Lifton MA, Beaudry KR, Manson K, Philippon V, Xu L, Maecker HT, Mascola JR, Panicali D, Nabel GJ, Letvin NL: Dysfunction of simian immunodeficiency virus/simian human immunodeficiency virus-induced IL-2 expression by central memory CD4+ T lymphocytes. J Immunol. 2005 Apr 15;174(8):4753-60.PubMed
Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer JA, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE: Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998 Apr 1;160(7):3610-6.PubMed
品牌介绍
EXBIO的优质抗体（GMP）目录 ＃产品名称克隆Reg。状态应用数量价钱ED7660SingleFlowEx CD71 APC新MEM-75RUO（GMP）足球俱乐部1毫升440.00美元ED7114SingleFlowEx CD25太平洋蓝™新MEM-181RUO（GMP）足球俱乐部1毫升440.00美元ED7252SingleFlowEx CD235a FITC新JC159RUO（GMP）足球俱乐部1毫升360.00美元ED7116SingleFlowEx CD25 PE新MEM-181RUO（GMP）足球俱乐部1毫升440.00美元ED7605SingleFlowEx CD138太平洋蓝™新MI15RUO（GMP）足球俱乐部1毫升440.00美元ED7165SingleFlowEx CD71 APC-Cy™7新MEM-75RUO（GMP）足球俱乐部1毫升500.00美元ED7228SingleFlowEx CD1a APC新SK9RUO（GMP）足球俱乐部1毫升440.00美元ED7115SingleFlowEx CD25 FITC新MEM-181RUO（GMP）足球俱乐部1毫升360.00美元ED7247SingleFlowEx CD305 PE新NKTA255RUO（GMP）足球俱乐部1毫升440.00美元ED7615SingleFlowEx CD235a PE-Cy™7新JC159RUO（GMP）足球俱乐部1毫升500.00美元ED7117SingleFlowEx CD25 APC新MEM-181RUO（GMP）足球俱乐部1毫升440.00美元ED7215SingleFlowEx CD103 APC新Ber-ACT8RUO（GMP）足球俱乐部1毫升440.00美元ED7219SingleFlowEx CD138 FITC新MI15RUO（GMP）足球俱乐部1毫升360.00美元ED7224SingleFlowEx CD138 PerCP-Cy™5.5新MI15RUO（GMP）足球俱乐部1毫升600.00美元ED7164SingleFlowEx CD71 Alexa Fluor系列700新MEM-75RUO（GMP）足球俱乐部1毫升440.00美元ED7253SingleFlowEx CD235a PE新JC159RUO（GMP）足球俱乐部1毫升440.00美元
联络我们
